49* Probing the oligomeric state of CFTR

Determining the Active Oligomeric State of SecA

LOGICOIL - multi-state prediction of coiled-coil oligomeric state

MOTIVATION The coiled coil is a ubiquitous α-helical protein-structure domain that directs and facilitates protein-protein interactions in a wide variety of biological processes. At the protein-sequence level, the coiled coil is readily recognized via a conspicuous heptad repeat of hydrophobic and polar residues. However, structurally coiled coils are more complicated, existing in a wide range ...

The oligomeric state of the active Vps4 AAA ATPase.

The cellular ESCRT (endosomal sorting complexes required for transport) pathway drives membrane constriction toward the cytosol and effects membrane fission during cytokinesis, endosomal sorting, and the release of many enveloped viruses, including the human immunodeficiency virus. A component of this pathway, the AAA ATPase Vps4, provides energy for pathway progression. Although it is establis...

Probing an Open CFTR Pore with Organic Anion Blockers

The cystic fibrosis transmembrane conductance regulator (CFTR) is an ion channel that conducts Cl- current. We explored the CFTR pore by studying voltage-dependent blockade of the channel by two organic anions: glibenclamide and isethionate. To simplify the kinetic analysis, a CFTR mutant, K1250A-CFTR, was used because this mutant channel, once opened, can remain open for minutes. Dose-response...

Probing the QCD Equation of State ∗

We propose a novel quasiparticle interpretation of the equation of state of deconfined QCD at finite temperature. Using appropriate thermal masses, we introduce a phenomenological parametrisation of the onset of confinement in the vicinity of the phase transition. Lattice results of bulk thermodynamic quantities are well reproduced, the extension to small quark chemical potential is also succes...